Indian Journal of Pharmacy and Pharmacology
Official Publication of Innovative Education and Scientific Research Foundation
Official Publication of Innovative Education and Scientific Research Foundation
Print ISSN: 2393-9079
Online ISSN: 2393-9087
CODEN : IJPPTK
Indian Journal of Pharmacy and Pharmacology (IJPP) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award programs. With more...Review Article
Author Details :
Volume : 1, Issue : 1, Year : 2014
Article Page : 1-17
Abstract
A simple, precise and rapid HPLC method has been developed for the simultaneous determination of Efavirenz, Tenofovir and Emtricitabine in pharmaceutical dosage form. The method was carried out using Zorbax C8 column (150 mm x 4.6 mm, 5 µm) and mobile phase comprised of mixture of dilute orthophosphoric acid solution pH 2.4±0.02 as buffer and acetonitrile in the ratio of 70:30 v/v and degassed under ultrasonication. The flow rate was 1.0 mL/min and the effluent was monitored at 252 nm. The retention times of Efavirenz, Tenofovir and Emtricitabine were 1.81 min, 2.80 min and 7.30 min respectively. The method was validated in terms of linearity, precision, accuracy, specificity, limit of detection, limit of quantitation and by performing recovery study. Linearity was in the range of 600.13 to 1800.39 µg/mL for Efavirenz, 300.54 to 900.44 µg/mL for Tenofovir and 200.46 to 601.38 µg/mL for Emtricitabine respectively. The percentage recoveries of all the three drugs were ranging from 98.2 to 101.9 for Efavirenz, 99.7 to 101.9 for Tenofovir and 98.7 to 101.6 for Emtricitabine respectively from the tablet formulation. The proposed method is suitable for the routine quality control analysis of simultaneous determination of Efavirenz, Tenofovir and Emtricitabine in bulk and pharmaceutical dosage form.
How to cite : Rao P V D A A L, Stability-Indicating RP-HPLC Method for the Simultaneous Estimation of Efavirenz, Tenofovir and Emtricitabine in Pharmaceutical Formulations. Indian J Pharm Pharmacol 2014;1(1):1-17
This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
