Indian Journal of Pharmacy and Pharmacology
Official Publication of Innovative Education and Scientific Research Foundation
Official Publication of Innovative Education and Scientific Research Foundation
Print ISSN: 2393-9079
Online ISSN: 2393-9087
CODEN : IJPPTK
Indian Journal of Pharmacy and Pharmacology (IJPP) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award programs. With more...Original Article
Author Details :
Volume : 3, Issue : 3, Year : 2016
Article Page : 146-151
Abstract
A simple, accurate and precise RP-HPLC method was developed for the estimation of Aceclofenac and Rabeprazole Sodium in the bulk and marketed formulation. The method was performed by using the mobile phase containing Methnol, Water and Acetonitrile (60:30:10 v/v), pH- 6.14 was found to be most suitable for RP-HPLC as it showed sharp peak with symmetry and significant reproducible retention time for Aceclofenac (4.549 min) along with Rabeprazole Sodium (6.048 min). From the results of system suitability parameters, it was observed that the peak was sharp and symmetrical with satisfactory capacity factor and column efficiency. From the developed methods, percent content of Aceclofenac and Rabeprazole Sodium was found to be 99.97%, 99.91% and 99.94% 99.6%, 99.8% and 94.45% respectively by RP-HPLC. The method was validated by parameters like accuracy, precision, specificity, linearity, ruggedness and robustness. The results indicated that, the method is simple, accurate, precise, rugged. The method described enables to the quantification of Aceclofenac and Rabeprazole Sodium. The advantages lie in the simplicity of sample preparation and the low costs of reagents used. The proposed HPLC conditions ensure sufficient resolution and the precise quantification of the compounds. Results from statistical analysis of the experimental results were indicative of satisfactory precision and reproducibility. Hence, this HPLC method can be used for routine drug analysis
How to cite : Adhao V S, RP-HPLC Method development and validation for the simultaneous estimation of Aceclofenac and Rabeprazole Sodium in the bulk and marketed formulation. Indian J Pharm Pharmacol 2016;3(3):146-151
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