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Development and Validation of Lercanidipine Hydrochloride and Atenolol by Using RP-HPLC and UV Spectroscopy

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Review Article

Author Details : Priyadarshani S. Bansode, Mahendra Singh Ranawat, Ravindra Kamble, Chetan Singh Chauhan

Volume : 1, Issue : 1, Year : 2014

Article Page : 37-41

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Abstract

 Lercanidipine hydrochloride (LCD), a dihydropyridine calcium channel blocker is used in the treatment of hypertension. Atenolol (ATN), a cardio selective β-blocker is used as   antihypertensive, antianginal and antiarrhythmic. The proposed method was development and validation of UV-Spectrophotometric and RP-HPLC for determination in bulk and tablet dosage form. In UV Spectroscopic method, LCD showed ?_max at 237 nm in 50% v/v acetonitrile. The RP-HPLC analysis was performed by using a mobile phase of methanol: water (95:5 v/v), at a flow rate of 1.0 ml/min. By comparing validation results of both the methods, UV-spectrophotometric method is found to be simple, economical and rapid and RP-HPLC method were found to more precise, accurate, rugged and robust.

 

How to cite : Bansode P S, Ranawat M S, Kamble R, Chauhan C S, Development and Validation of Lercanidipine Hydrochloride and Atenolol by Using RP-HPLC and UV Spectroscopy. Indian J Pharm Pharmacol 2014;1(1):37-41

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