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Reverse phase high performance liquid chromatography and UV-Visible method for the simultaneous estimation of gemifloxacin

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Original Article

Author Details : Senthilraja M*

Volume : 6, Issue : 2, Year : 2019

Article Page : 60-64

https://doi.org/10.18231/j.ijpp.2019.014

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Abstract

A novel UV-Visible and reversed phase High Performance Liquid Chromatography method has been developed and validated for the simultaneous analysis of gemifloxacin and their formulation. The UV-Visible method was carried with folin reagent and reverse Phase HPLC method with acetonitrile: phosphate buffer (40:60%v/v) mobile phase, having a pH of 3.0 at a wavelength of 272 nanometer. The linearity range starts from 2 to10?g/ml and 1 to 5?g/ml, percentage recovery was found to be 98.55% and 99.68%, limit of detection 0.2651?g and 0.1588?g and 0.8033?g and 0.4811?g for the UV-Visible and RP-HPLC methods respectively.

Keywords: Reversed phase-HPLC, UV-Visible, Gemifloxacin, Folin-Ciocalteu phenol reagent, Phosphate buffer.

How to cite : Senthilraja M, Reverse phase high performance liquid chromatography and UV-Visible method for the simultaneous estimation of gemifloxacin. Indian J Pharm Pharmacol 2019;6(2):60-64

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